Binding of dihydropyridine calcium antagonists to membranes from human skeletal muscle.
نویسندگان
چکیده
منابع مشابه
Calcium binding to extracellular sites of skeletal muscle calcium channels regulates dihydropyridine binding.
The binding of dihydropyridine (PN200-110) to skeletal muscle microsomes (which were 84% sealed inside-out vesicles) was not influenced by the addition of calcium or magnesium nor by addition of their chelators (EDTA or EGTA) unless the vesicles were pretreated with the calcium-magnesium ionophore A23187 and EDTA to remove entrapped cations. Separation of inside-out vesicles from right-side-out...
متن کاملA Monoclonal Antibody to the , 6 Subunit of the Skeletal Muscle Dihydropyridine Receptor Immunoprecipitates the Brain w - Conotoxin GVIA Receptor
Antibodies against the subunits of the dihydropyridine-sensitive L-type calcium channel of skeletal muscle were tested for their ability to immunoprecipitate the high affinity (ICd = 0.13 nM) "'I-w-conotoxin GVIA receptor from rabbit brain membranes. Monoclonal antibody VD21 against the B subunit of the dihydropyridine receptor from skeletal muscle specifically immunoprecipitated up to 86% of t...
متن کاملA QUANTUM CHEMICAL STUDY OF STRUCTURE -ACTIVITY RELATIONSHIPS OF DIHYDROPYRIDINE CALCIUM ANTAGONISTS
Quantum chemical (MNDo)caleulations have beenused toelucidate the molecular properties and structure - activity relationships of dihydropyridine (DHP) type calcium antagonists. There is a good corielation between the net atomic charges on various atoms of the 4 - phenyl ring of dihydropyridines and pharmacological activity. .Also, activity decreases with increasing free rotation of the phen...
متن کاملDihydropyridine and phenylalkylamine receptors associated with cardiac and skeletal muscle calcium channels are structurally different.
We have purified putative L-type Ca2+ channels from chick heart by virtue of their associated high affinity receptors for the Ca2+ channel effectors, dihydropyridines (DHPs), and phenylalkylamines (PAAs). A peptide of 185,000-190,000 daltons was found to comigrate with the peak of DHP binding activity during purification through two successive cycles of lectin affinity chromatography and sucros...
متن کاملReduced calcium sensitivity of dihydropyridine binding to calcium channels in spontaneously hypertensive rats.
To explore the role of calcium channels in hypertension, dihydropyridine ([3H]PN200-110) binding to heart, brain, and skeletal muscle microsomes of 4-, 8- and 15-week-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats was measured. At a constant Ca2+ ion concentration (pCa 3.0), maximal binding (Bmax) of dihydropyridine binding to heart and brain microsomes was significantly ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- General physiology and biophysics
دوره 7 3 شماره
صفحات -
تاریخ انتشار 1988